Aplicaciones de la citometría de flujo en hematología

María Luisa Abad-Castillo; Gloria Patricia Angamarca-Angamarca; Franklin Esteban Martínez-Saltos; Vanessa Margarita Naranjo-Torres

Authors

Abad-Castillo María Luisa Máster en Gestión de la Seguridad Clínica del Paciente y Calidad en la Atención Sanitaria, Bioquímica Farmacéutica. Palanda, Ecuador. https://orcid.org/0009-0004-2577-4675
Angamarca-Angamarca Gloria Patricia Bioquímica Farmacéutica. Santa Rosa, Ecuador. https://orcid.org/0009-0001-0064-9673
Martínez-Saltos Franklin Esteban Licenciado en Laboratorio Clínico, Universidad Técnica de Ambato. Ambato, Ecuador. https://orcid.org/0000-0001-5687-9622
Naranjo-Torres Vanessa Margarita Licenciado en Laboratorio Clínico, Universidad Técnica de Ambato. Ambato, Ecuador. https://orcid.org/0009-0000-5340-3397

Keywords:

biological process, phenotypes, cells, science

Abstract

DOI: https://doi.org/10.46296/gt.v8i15.0213

The processes that occur inside cells are extremely complex and multifaceted, and are regulated by a dynamic and constantly changing intracellular signaling system. In order to gain a deeper and more detailed understanding of the various biological processes involved, the technique known as flow cytometry is used. This methodology allows us to determine the phenotypic characteristics of cells by measuring their dimensions and carrying out a simplified analysis of their internal composition. Flow cytometry is a methodology used in the field of haematology, which is dedicated to the detailed study of blood, with the aim of classifying the different cells that compose it, based on criteria such as their size and the complexity of their structure. Since its creation in 1976, flow cytometry has evolved considerably and has incorporated a variety of new functions that extend its usefulness in various fields of science and medicine. At this moment, it has the capacity to classify cells using different methods based on fluorescence, which allows for a detailed analysis of cell viability, as well as exploring other relevant aspects related to them. Flow cytometry is a technique that is widely used in the field of biomedical research, standing out for its ability to analyze cellular characteristics accurately. However, it is worth mentioning that, in addition to its application in research, this methodology also plays a significant role in the practice of clinical diagnosis, thus contributing to the identification and analysis of various conditions and diseases in patients.

Keywords: biological process, phenotypes, cells, science.

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References

Láser L, Hernández-Reyes H. Avances y aplicación clínica de la citometría hemática automatizada Advances and clinical application of automated hematic cytometry. Inmunología y Hemoterapia [Internet]. 2013;29(1):24–39. Available from: http://scielo.sld.cu

Vianed D, Suárez M, Lázaro L, Del Valle Pérez O, Gabriela L, Domínguez D, et al. Metodología y aplicaciones de la citometría de flujo para el inmunofenotipaje de las leucemias agudas Methodology and applications of flow cytometry for immunophenotyping of acute leukemias. Revista Cubana de Hematol, Inmunol y Hemoter [Internet]. 2015;31(3):242–53. Available from: http://scielo.sld.cu

March Rossello G, Eiros Bouza J. CITOMETRÍA DE FLUJO: FUNDAMENTO, INSTRUMENTACIÓN Y APLICACIONES EN MICROBIOLOGÍA CLÍNICA. Revista Electronica de Biomedicina. 2012;3:45–53.

Gala De Pablo J, Lindley M, Hiramatsu K, Goda K. High-Throughput Raman Flow Cytometry and beyond. Acc Chem Res. 2021 May 4;54(9):2132–43.

Adan A, Alizada G, Kiraz Y, Baran Y, Nalbant A. Flow cytometry: basic principles and applications. Crit Rev Biotechnol. 2017 Feb 17;37(2):163–76.

Gascue A, Merino J, Paiva B. Flow Cytometry. Vol. 32, Hematology/Oncology Clinics of North America. W.B. Saunders; 2018. p. 765–75.

Rossi A, Pacella I, Piconese S. Rna flow cytometry for the study of t cell metabolism. Vol. 22, International Journal of Molecular Sciences. MDPI AG; 2021.

Triana Marrero Y, Marsan Suarez V. Aplicaciones de la citometría de flujo en el estudio de los síndromes linfoproliferativos crónicos. Revista Cubana de Hematología [Internet]. 2020;36. Available from: http://orcid.org/0000-0003-3391-0767

Soler Noda G. La citometría de flujo en el estudio de las citopenias inmunes. Revista Cubana de Hematología, Inmunología y Hemoterapia. 2019;35(1).

Herrera Macera SP, Boada Rodríguez LE, Colamarco Navas WG, Boada Rodríguez KG. Aplicación de la citometría de flujo en el diagnóstico de inmunopatía. RECIAMUC. 2019 Jan 30;3(1):377–97.

Ramírez Orellana M. Citometría de flujo: qué puede aportar al diagnóstico hematológico en pediatría. Anales de Pediatria Continuada. 2012 Sep;10(5):282–5.

Herbig M, Tessmer K, Nötzel M, Nawaz AA, Santos-Ferreira T, Borsch O, et al. Label-free imaging flow cytometry for analysis and sorting of enzymatically dissociated tissues. Sci Rep. 2022 Dec 1;12(1).

Juan Otero Europa Azucena González-Navarro M, Biología L. APLICACIONES CLÍNICAS DE LA CITOMETRÍA DE FLUJO. EDUCACIÓN CONTINUADA EN EL LABORATORIO CLÍNICO. 2014;17:62–70.

Del Principe MI, De Bellis E, Gurnari C, Buzzati E, Savi A, Consalvo MAI, et al. Applications and efficiency of flow cytometry for leukemia diagnostics. Expert Rev Mol Diagn. 2019 Dec 2;19(12):1089–97.

Kalina T, Abraham RS, Rizzi M, van der Burg M. Editorial: Application of Cytometry in Primary Immunodeficiencies. Front Immunol. 2020 Mar 19;11.